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There is no way of knowing the conditions prior to treatment, and changes may be due to the burn. In column D, there is a single treatment unit and a single control unit. Perhaps you see a change occur in the burned area but not the control area. The change could be caused by the burn or there may be some other factor that differentially affects the treatment area compared to the control. The burn unit, for example, could have a slightly lower water table than the control unit. Other factors such as disease. Any of these factors could be the cause of observed differences. In the last two columns, the treatment and control are replicated in space; thus there is a possibility of attributing differences to the treatment.
Since ecological systems are variable, the example in column E with three replicates may have inadequate statistical power to detect differences. The differences due to the treatment may be hidden by differences that occur due to other factors. The larger number of replicates in column F greatly increases the likelihood of detecting treatment differences due to the higher statistical power associated with 8 replicates as compared to 3 replicates.
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