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Effect of experimental treatment on housekeeping gene expression:. Journal of experimental when, ; 55 rfal, — Journal of Experimental Botany, ; 54— Cucumber the following to cite this article: The number of patient that had 7bp cell did not show any expression change in HOXB1 gene. A indeed Iranian kindred with overall 5 affected individuals along with their unaffected siblings and parents were needed. Journal of experimental botany, ; 55— Journal of Experimental Fuse, ; 54— Copy the following to cite this article: The number of patient that had 7bp eel did not show any expression change in HOXB1 gene.

Adenine phosphoribosyl dawht aprt and tubulin may also be used. Many studies on housekeeping gene expression mainly deal with human tissues, bacteria and viruses Volkov et al. Consequently, choosing an internal control is too important to gene expression quantify Langer et al. The use of GAPDH as the internal standard could be a valuable alternative to quantify genes of interest, keeping in mind that it could reduce the variations of expression Nicot et al. Materials and Methods Sample Collection 36 blood samples were obtained from patients randomly. Clinical and pathological data of patients were collected.

The experiment protocols and informed consent forms were approved Mdet the Human Studies Committee at the Affiliated the Medical Science University. Synthesized cDNA was then checked spectrophotometrically to estimate its concentration. Forward and reverse primers sequences have shown in table Meet real girls in marv dasht. Melting girlss analysis was performed to verify specificity of PCR products. Statistical Analysis The pairwise fixed reallocation randomization test with iterations in the REST software was used to determine the significances. Statistical analyses of demographic and clinical data were performed using SPSSv.

Chi-square and independent t tests were used for testing the relationship between categorical variables. Results General Statistical Information Data have been analyzed based on the information taken from questionnaires, interviews, and clinical and laboratory tests. Expression level of HOXB1 gene have shown in figure 1. B A section of Sanger sequencing Chromatograms for the homozygous mutation below and wild type top are depicted and demonstrate the insertion in the box.

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Discussion Facial paresis is a rare, hereditary congenital and nonprogressive. From till now only 3 cases of the disease have been reported in the world therefore there is no definition specific prevalence of this disease and its hereditary have been reported as autosomal recessive Uyguner et al. Facial paresis due to the similarity of symptoms named Moebius syndrome. The first report was done by Goddard et al in showed a nonsense mutation in HOXB1 gene in mice caused impaired development of facial nerve but no similar cases were reported in human Goddard et al. The role of HOXB1 gene and its relationship with other developmental genes had been Meet real girls in marv dasht by Carolyn et al in Slupsky et al.

The results of Carolyn et al indicated that the structure of the protein product has been quite stable. This mutation lies in the DNA-binding homeodomain of HOXB1 located in between and residues, which is highly conserved among several species Sahin et al. Michielse Meet real girls in marv dasht al in using linkage analysis method find HCFP 3q21 in a Pakistani family and did not find result of on search for other candidate genes Michielse et al. Another homozygous missense mutation in HOXB1, also affecting arginine [c.

ArgHis ] was found in a Turkish girl with bilateral facial weakness, left esotropia, left ptosis, and midface retrusion. Auditory brainstem response test revealed normal results and MRI scans showed no structural anomalies MacKinnon et al. Clinical features of all affected individuals including hearing loss, midface retrusion, lagophthalmos, oral dysfunction, swallowing difficulties, dysarthria, and speech delay suggested the diagnosis of HCFP Vogel Meet real girls in marv dasht al. The results of Vogel et al. The number of patient that had 7bp mutation did not show any expression change in HOXB1 gene.

Acknowledgement We gratefully thank the Medical genetics research center of the Shahid Sadoughi University of Medical Sciences Meet real girls in marv dasht the patients and their families for cooperation in this study. Conflict of interest The authors declare no conflict of interest. A Meet real girls in marv dasht with hereditary congenital facial paresis and a brief review of the literature. Clinical Dysmorphology, ; 19 4— Homeobox genes and disease. Plant Molecular Biology Reporter, ; 20 4— Journal of experimental botany, ; 55— Mice with targeted disruption of Hoxb-1 fail to form the motor nucleus of the VIIth nerve.

Development Cambridge, England; 10— Ophthalmology, ; 7— Development, ; 19— Refinement of the locus for hereditary congenital facial palsy on chromosome 3q21 in two unrelated families and screening of positional candidate genes. European Journal of Human Genetics, ; 14 12— The Mystery of the Missing Smile. Science, ;— Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. The steps measured 6. New theories, however, suggest that the shallow risers allowed visiting dignitaries to maintain a regal appearance while ascending. The top of the stairways led to a small yard in the north-eastern side of the terrace, opposite the Gate of All Nations.

Grey limestone was the main building material used at Persepolis. After natural rock had been leveled and the depressions filled in, the terrace was prepared. Major tunnels for sewage were dug underground through the rock. A large elevated water storage tank was carved at the eastern foot of the mountain. Professor Olmstead suggested the cistern was constructed at the same time that construction of the towers began. The uneven plan of the terrace, including the foundation, acted like a castle, whose angled walls enabled its defenders to target any section of the external front. Diodorus Siculus writes that Persepolis had three walls with ramparts, which all had towers to provide a protected space for the defense personnel.

The first wall was 7 metres 23 feet tall, the second, 14 metres 46 feet and the third wall, which covered all four sides, was 27 metres 89 feet in height, though no presence of the wall exists in modern times. Function[ edit ] The function of Persepolis remains rather unclear. It was not one of the largest cities in Persia, let alone the rest of the empire, but appears to have been a grand ceremonial complex, that was only occupied seasonally; it is still not entirely clear where the king's private quarters actually were. Until recent challenges, most archaeologists held that it was especially used for celebrating Nowruzthe Persian New Year, held at the spring equinoxand still an important annual festivity in modern Iran.

The Iranian nobility and the tributary parts of the empire came to present gifts to the king, as represented in the stairway reliefs. He stormed the " Persian Gates ", a pass through modern-day Zagros Mountains. There Ariobarzanes of Persis successfully ambushed Alexander the Great's army, inflicting heavy casualties. After being held off for 30 days, Alexander the Great outflanked and destroyed the defenders. Ariobarzanes himself was killed either during the battle or during the retreat to Persepolis. Some sources indicate that the Persians were betrayed by a captured tribal chief who showed the Macedonians an alternate path that allowed them to outflank Ariobarzanes in a reversal of Thermopylae.

After several months, Alexander allowed his troops to loot Persepolis. Alexander the Great ordering Persepolis to be set on fire; Italian plate, although it may be a depiction of the burning of Rome by Nero. Around that time, a fire burned "the palaces" or "the palace". Scholars agree that this event, described in historic sources, occurred at the ruins that have been now re-identified as Persepolis. From Stolze's investigations, it appears that at least one of these, the castle built by Xerxes I, bears traces of having been destroyed by fire. The locality described by Diodorus Siculus after Cleitarchus corresponds in important particulars with the historic Persepolis, for example, in being supported by the mountain on the east.

It is believed that the fire which destroyed Persepolis started from Hadish Palace, which was the living quarters of Xerxes Iand spread to the rest of the city. Many historians argue that, while Alexander's army celebrated with a symposiumthey decided to take revenge against the Persians. The Book of Arda Wiraza Zoroastrian work composed in the 3rd or 4th century, describes Persepolis' archives as containing "all the Avesta and Zendwritten upon prepared cow-skins, and with gold ink", which were destroyed. Indeed, in his Chronology of the Ancient Nations, the native Iranian writer Biruni indicates unavailability of certain native Iranian historiographical sources in the post-Achaemenid era, especially during the Parthian Empire.

People say that, even at the present time, the traces of fire are visible in some places. After the fall of the Achaemenid Empire[ edit ] Ruins of the Western side of the compound at Persepolis.


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