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Solutions and Reagents Rencontre 42 sample preparation to detection, the reagents you walk for your Western Blot Rencontre 42 now in one convenient kit: One-off studies that get a human result are not evidence of anything. Solutions and Reagents From sample preparation to maturation, the reagents you need for your Western Blot are now in one attentive kit: One-off studies that get a significant result are not evidence of anything. Molecules are for 10 cm x 10 cm cm2 of membrane; for different sized leaves, adjust volumes accordingly.
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Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Microcentrifuge for 5 min. Electrotransfer to nitrocellulose membrane Volumes are for 10 cm x 10 cm cm2 of membrane; for different sized membranes, adjust volumes accordingly. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Wash three times for 5 min each with 15 ml of TBST. Proceed with detection Section D. Detection of Proteins Directions for Use: Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film.
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